Natural Preservation of Chili Garlic Sauce Using ε-Polylysine, Nisin & Natamycin
✅ Solution:
A natural antimicrobial blend was developed using:
ε-Polylysine (0.1%): Broad-spectrum antibacterial, especially against E. coli, S. aureus
Nisin (0.05%): Inhibits Gram-positive spoilage and pathogenic bacteria (L. monocytogenes)
Natamycin (0.015%): Effective antifungal to prevent mold and yeast growth on surface and in particulates
Application method:
All preservatives were added during paste mixing & homogenization phase. Product was hot-filled at 85°C and sealed in glass jars
Results:
| Parameter |
Control (No preservatives) |
Natural Blend |
| Shelf life (ambient) |
20 days |
≥ 180 days |
| Mold/Yeast growth (day 30) |
Detected |
Not detected |
Pathogen growth
(L. mono, S. aureus) |
Present |
Inhibited |
| Sensory impact |
Slight acid/salty aftertaste |
No difference detected |
Region: Exported to Southeast Asia, EU, and Middle East markets with tight additive restrictions
Benefits to Customer:
Clean label: All preservatives are natural, GRAS, and consumer-friendly
Extended shelf life without refrigeration
No impact on color, taste, or aroma
Complied with EU Regulation (EC) No 1333/2008 and GB2760-2022
✅ Recommended Ingredients for Sauces:
ε-Polylysine – Broad-spectrum antimicrobial
Nisin – Heat-stable, ideal for acidic sauces
Natamycin – Surface mold & yeast control
Optional: Rosemary Extract or Tea Polyphenols for antioxidant protection in oil-based sauces