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    • ε-Polylysine
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      Natamycin
    • Rosemary Extract
      Rosemary Extract
    Dipping Sauce
    Sauce & Seasoning

    Product: Chili-Based Dipping Sauce with Sesame Paste, Garlic, and Fermented Bean Paste (pH \~4.3, aw > 0.94)

    Solution: ε-Polylysine 0.1%, Natamycin 0.015%, Rosemary Extract 0.05% (Optional Antioxidant for Oil Phase)

    Region: China, Export Markets (Clean Label Preservation Project)

    Combination: ε-Polylysine and Natamycin used for microbial control, Rosemary extract added for antioxidant benefits in the oil phase


    Multi-Target Microbial Control in Spicy Dipping Sauce (Hot Pot Sauce)



    Introduction: Spicy dipping sauces, especially for hot pot dishes, are popular condiments with a rich, complex flavor profile. However, their high moisture content, moderate pH (~4.3), and exposure to air after opening make them vulnerable to spoilage, especially by mold, yeast, and Bacillus species. Furthermore, export markets often require products with natural preservation methods, avoiding synthetic additives. This case investigates the application of ε-Polylysine, Natamycin, and Rosemary Extract as a clean-label preservation strategy for chili-based dipping sauces.



    Issues:

    Microbial Growth: Dipping sauces are susceptible to mold, yeast, and Bacillus growth, which can lead to product spoilage within 7–10 days of opening.

    Spoilage After Opening: Even with refrigerated storage, the sauce often shows signs of microbial growth after just 1 week.

    Export Market Requirements: Consumers and regulations in export markets increasingly demand natural preservatives free from synthetic chemicals.



    Solution: 
    To extend the shelf life and prevent spoilage, the following ingredients were added:
     

    ε-Polylysine 0.1%: A broad-spectrum antimicrobial, ε-Polylysine was included to control bacterial contamination while preserving the clean-label status.

    Natamycin 0.015%: Specifically targets mold and yeast, ensuring that these microorganisms do not thrive in the sauce.

    Rosemary Extract 0.05% (Optional): An antioxidant that helps stabilize the oil phase, preventing oxidative rancidity and maintaining flavor integrity.

    The antimicrobial agents were added during the blending process, before thermal treatment, ensuring even distribution throughout the product.



    Results:

    Shelf Stability: The dipping sauce remained stable at room temperature for 6 months without any visible microbial growth, even after opening.

    Extended Shelf Life After Opening: No microbial growth was observed after 30 days of storage in a refrigerated environment, far beyond the typical 7–10 days before spoilage.

    Flavor and Color Maintenance: The sauce maintained its original flavor and vibrant color, even under accelerated aging conditions, ensuring the product's appeal over time.

    Clean Label Compliance: The solution met the demand for natural preservatives, making it suitable for export markets with stringent clean-label requirements.



    Conclusion: The combination of ε-Polylysine, Natamycin, and Rosemary Extract provides an effective, clean-label solution for preserving the quality and shelf life of chili-based dipping sauces. This natural preservation system inhibits mold, yeast, and bacterial growth, extends the shelf life from days to months, and maintains the sauce’s flavor, texture, and appearance, making it an ideal solution for domestic and export markets alike.
     
     
     
    Natural Protective Solutions
    Rosemary Extract
    Rosemary Extract
    A Natural Antioxidant and Antimicrobial for Food, Cosmetics, and Nutraceuticals
    Rosemary Extract is a natural plant-derived antioxidant and antimicrobial agent, primarily standardized to carnosic acid and carnosol. It is widely used in food preservation, cosmetics, and nutritional supplements to delay oxidation, prevent rancidity, an
    View details
    nisin
    Nisin
    A Natural, Heat-Stable Food-Grade Antimicrobial Peptide for Extended Shelf Life
    Nisin is a natural antimicrobial peptide produced by Lactococcus lactis. It is widely used as a food preservative to inhibit Gram-positive bacteria and spore-formers, including Clostridium botulinum and Listeria monocytogenes. With excellent heat stabilit
    View details
    ε-Polylysine
    ε-Polylysine
    Natural, Biodegradable Antimicrobial Preservative for Food and Personal Care Products
    ε-Polylysine is a natural antimicrobial peptide produced through microbial fermentation. It is widely used as a food preservative due to its broad-spectrum antimicrobial activity, excellent safety profile, and biodegradability.
    View details
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